Prevalencia del Virus de la Necrosis Hipodérmica y Hematopoyética Infecciosa (IHHNV) en Litopenaeus vannamei de producción comercial mediante PCR en “Empagran” en la provincia del Guayas.

Abstract

This project took place in the genetics laboratory belonging to the research and development department in the molecular biology area of Empagran S.A, which is located at km 19.5 via a la costa in the province of Guayas. There was a total of 100 samples of Litopenaeus vannamei, which were distributed in 3 stages: postlarvae, maturation animals and shrimp farm animals. The goal of the project was to evaluate the presence of infectious hypodermic and hematopoietic necrosis virus and correlate it with the different variables chosen (temperature, salinity, pH, oxygen and saturation). The IQ2000 IHHNV kit (nested) was used for PCR as an analysis method. At the end of the project and performing the analysis, it was concluded that the prevalence of IHHNV was low and that the variables did influence the presence of the virus with the exception of saturation. As for the shrimp farmers and people involved in this field, a better control is recommended and also opt for more sensitive and accurate tests for disease detection.